Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit |
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| Authors: | Mary Woodhead Mark A. Taylor Howard V. Davies Rex M. Brennan Ronnie J. McNicol |
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| Affiliation: | (1) Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland, UK |
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| Abstract: | Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction. |
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| Keywords: | RNA isolation blackcurrant fruit Ribes nigrum |
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