Rapid Detection of Virulence-Associated Genes in Environmental Strains of Vibrio cholerae by Multiplex PCR |
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Authors: | Praveen Kumar Wilson A. Peter Sabu Thomas |
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Affiliation: | (1) Cholera and Environmental Microbiology Lab, Department of Molecular Microbiology, Rajiv Gandhi Center for Biotechnology, Trivandrum, Kerala, India; |
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Abstract: | Vibrio cholerae, the causative agent of cholera is ubiquitously distributed in aquatic environment particularly in coastal waters, estuaries, and rivers. In the present investigation, a multiplex PCR assay was developed for the detection of virulence-associated genes (rtxA, tcpA, ctxA, hlyA, and sto) in environmental isolates of V. cholerae. A total of 90 strains isolated from different environmental sources were screened for the presence of virulence-associated genes. Our results showed that this method represents a simple, cost effective, and robust tool for rapid detection of virulence-associated genes. This multiplex PCR can be used for examining prevalence of virulence-associated genes and hence will be useful for better understanding of epidemiology of environmental V. cholerae. |
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