Identification of a wheat germ agglutinin-sensitive ATPase in yeast nuclei |
| |
Authors: | Eiko Tsuchiya Kazumi Hiraga Sakuzo Fukui Tokichi Miyakawa |
| |
Institution: | Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan. |
| |
Abstract: | We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the presence of WGA-binding materials around the nuclear pore under an electron microscope. Using WGA-agarose column chromatography of yeast nuclear extracts, a novel Mg2+-dependent ATPase was isolated. Its activity was highly sensitive to WGA and stimulated by Nonidet P-40 or phosphatidylserine. We suggest that the WGA-sensitive ATPase plays a role in yeast nuclear transport of DNA. |
| |
Keywords: | ATPase nuclear Nuclear transport Wheat germ agglutinin-binding protein (Saccharomyces cerevisiae) |
本文献已被 ScienceDirect 等数据库收录! |