| 亚硝酸与紫外线复合诱变原生质体选育产酶菌株 |
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| 引用本文： | 肖怀秋,兰立新,李玉珍.亚硝酸与紫外线复合诱变原生质体选育产酶菌株[J].生物技术,2006,16(5):40-41. |
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| 作者姓名： | 肖怀秋 兰立新 李玉珍 |
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| 作者单位： | 1. 湖南化工职业技术学院应用化学系,湖南,株洲,412004
2. 湖南农业大学食品科学技术学院,湖南,长沙,410128 |
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| 基金项目： | 长沙市科技项目资助(“高活性复合蛋白酶制剂的生产技术攻关研究”，No．K032035-12)~~ |
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| 摘 要： | 目的：筛选出一株稳定、高产的产中性蛋白酶菌株。方法：以突变株UV_(11)(原生质体紫外诱变得到)为出发菌株,在原生质体形成与再生最佳条件下制备原生质体并进行亚硝酸与紫外线复合诱变。结果：得到突变株Bacillus subtilis UN_(19),产酶活力从最初的378．97U/ml提高到3965．84U/ml。结论：亚硝酸与紫外线复合诱变原生质体是一种很好的诱变方式。
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| 关 键 词： | 枯草芽孢杆菌 原生质体 复合诱变 中性蛋白酶 |
| 文章编号： | 1004-311X（2006）05-0040-02 |
| 收稿时间： | 2006-04-28 |
| 修稿时间： | 2006-07-12 |
Compound Mutagenesis of HNO2 and UV of Protoplast on Screening of Enzyme - producing Strain |
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| XIAO Huai-qiu,LAN Li-xin,LI Yu-zhen.Compound Mutagenesis of HNO2 and UV of Protoplast on Screening of Enzyme - producing Strain[J].Biotechnology,2006,16(5):40-41. |
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| Authors: | XIAO Huai-qiu LAN Li-xin LI Yu-zhen |
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| Institution: | 1. Department of Application Chemical, College of Hunan Chemical Industry and Vocational Institute, ZHuzhou 412004, China; 2. College of Food Science and Technology, Hunan Agricultural University , Changsha 410128, China |
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| Abstract: | Objective:A stable,high-yield neutral proteinase-producing strain can be screened.Methods:Compound mutagenesis of UV and HNO_2 on protoplast from Bacillus subtiIis UV_(11) was launched and protoplast was prepared at its optimal formation and regeneration conditions.Re- suits:A mutant strain Bacillus subtilis UN_(19) was obtained and its neutral proteinase-producing capacity was improved from 378.97U/ml to 3965.84U/ml.Condusion:The method of compound mutagenesis of HNO_2 and UV of protoplast for screening of high producing strain was con- firmed as a good way. |
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| Keywords: | Bacillus subtilus protoplast compound mutagenesis neutral proteinase(NPR) |
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