东北马鹿和东北梅花鹿F_1杂种精母细胞联会复合体分析

作者以界面铺张-硝酸银染色技术,对东北马鹿和东北梅花鹿的F_1可育杂种的精母细胞联会复合体进行亚显微观察及分析。在减数分裂前期,杂种鹿精母细胞中形成31条完整的常染色体联会复合体、一个端着丝粒染色体/中着丝粒染色体的三价体和XY双价体。这进一步证明,两种亲本鹿的染色体具有高度的同源性,其差别仅在于一个罗伯逊易位。三价体的顺式构型可能和杂种鹿的可育性有关。

Analysis of Synaptonemal Complexes in Spermatocytes of the Hybrid F1 Between Red Deer and Sika Deer

The fertile F1 hybrid between red deer (Cervus elaphus xanthopygus,2n=68) and sik?deer (C.nippon hortalorum,2n=66) has been reported in captivity as well as in nature.The diploid chromosome number is 67.The G-banding patterns of every chromosome of the sika deer and red deer are identical except for No.1 chromosome.According to the G-banding pattern,No.1 chromosome of sika deer corresponds to,the two acrocentric chromosomes of Ted deer,and therefore may involve a Robertsonian translocation.In order to confirm this suggestion,the meiosis of F1 hybrid has been examined by ele-ctromicroscopically.Thirty-one normal autosomal bivalents,an acrocentric/metacentric tri-valent and an XY pair of synaptonemal complex karyotype were observed.The proximal telomeric knobs of the two acrocentric elements were paired and projected in the same direction from the metacentric element.The configuration of the trivalent may be a prerequisite for normal disjunction and balanced gamete formation,therefore,it can be accounted for the high fertility of the F1 hybrid.