Structure of cDNA clones for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from a fern (Asplenium cataractarum rosenstock,aspleniaceae), and its comparison with those of various seed plants |
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Authors: | Noriaki Murakami Yoshiyuki Tanaka Kunio Takishima Yuzo Minobe Makoto Matsuoka Sei-Ichiro Kiyota Shin-ichi Hatanaka Katsuhiro Sakano |
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Affiliation: | (1) Botanical Gardens, Nikko, Faculty of Science, University of Tokyo, Hanaishi-cho 1842, Nikko, 321-14 Tochigi;(2) National Institute of Agrobiological Resources, Kamnondai, 305 Tsukuba Science City, Ibaraki;(3) National Defence Medical College, Tokorozawa, Namiki, 359 Saitama;(4) Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, 153 Tokyo |
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Abstract: | We isolated the small subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO SSu) from a fern,Asplenium cataractarum and determined its 34 N-terminal amino acid sequence. We obtained a cDNA clone that contains the entire coding region of the SSu from the same fern species, using synthetic oligonucleotide probes derived from the above amino acid sequence. It contains a 525 bp open reading frame capable of coding for a polypeptide with 174 amino acids, 31 bp 5′-and 206 bp 3′-noncoding regions. It was also elucidated that the precursor to the SSu contains a transit peptide of 53 amino acid residues and a mature protein of 121 residues. We compared the deduced amino acid sequence of the fern SSu with those of 11 other vascular plant species (including gymnosperms, monocots and dicots). As low as 55% homology was observed between those of a fern and seed plants. Constancy of the amino acid substitution rate in RuBisCO SSu was supported by our relative rate test. Amino acid substitution rate per year per site for RuBisCO SSu was calculated to be 0.81×10−9 assuming that the separation between pteridophytes and seed plants arose 380 million years ago. |
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Keywords: | Aspleniaceae Asplenium cataractarum cDNA Molecular evolution Ribulose-1, 5-bisphosphate carboxylase/oxygenase Small subunit |
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