Improved direct method for determination of keto acids by 2,4-dinitrophenylhydrazine |
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| Authors: | H Katsuki T Yoshida C Tanegashima S Tanaka |
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| Affiliation: | 1. Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605 014, India;2. Department of Biotechnology, Pondicherry University, Puducherry, 605 014, India;1. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China;2. Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China;3. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China;1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China;2. Department of Chemistry and Biochemistry, California State University, Los Angeles, CA 90032, USA;3. Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China |
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| Abstract: | An improved method was devised for the determination of keto acid by 2,4-dinitrophenylhydrazine with the omission of the extraction procedure with organic solvent. Measurement of the coloration after addition of alkali was conducted at a particular wavelength not affected by the proportion of cis and trans isomers of the hydrazone. This method was applied to the analysis of some aliphatic keto acids; almost complete recoveries were obtained. |
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