Caspase-2/NEDD-2 protease mediates execution of apoptosis in AK-5 tumor cells |
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Authors: | A Khar B V V Pardhasardhi C Varalakshmi A M Ali A L Kumari |
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Institution: | (1) Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India |
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Abstract: | AK-5 tumour cells have been shown to undergo apoptosis in vitro and in vivo. The efficient killing of tumour cells by necrosis
and apoptosis leads to spontaneous regression of the tumour. To investigate a possible involvement of caspase-2/Nedd-2 protease
in AK-5 apoptosis, we introduced Nedd-2 gene in antisense orientation and showed inhibition of tumour cell apoptosis. Similarly
introduction of the bcl-2 gene in tumour cells also inhibited the apoptotic programme. NK cells which have previously been
shown to be the effector cells also fail to induce apoptosis in Nedd-2 antisense and bcl-2 transfected clones whereas NK mediated
cytotoxic activity is not altered in the transfectants. These results suggest participation of Nedd-2 protease in the induction
of apoptosis in AK-5 cells leading to tumour regression.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | AK-5 apoptosis Nedd-2 protease tumour regression |
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