Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA |
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Authors: | Toshikazu Irie Yoichi Honda Takashi Watanabe Masaaki Kuwahara |
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Institution: | Wood Research Institute, Kyoto University, Japan. |
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Abstract: | The effects of carrier DNAs on the transformation of the basidiomycete Pleurotus ostreatus were analyzed. When lambda phage DNA was added to a transformation mixture containing protoplasts and CbxR vector plasmid, an increased number of drug-resistant transformants was observed on a screening plate containing 2 microg carboxin/ml. The highest efficiency (about 200 transformants/microg vector plasmid) was obtained by the addition of heat-denatured lambda DNA, which gave yields approximately 50-fold higher than the control experiment without a carrier DNA. To our knowledge, this is the first report on enhancement in transformation efficiency of fungal protoplasts by single strand carrier DNA. |
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