Transgenic regal pelargoniums that express the rolC gene from Agrobacterium rhizogenes exhibit a dwarf floral and vegetative phenotype |
| |
Authors: | Boase M R Winefield C S Lill T A Bendall M J |
| |
Institution: | (1) New Zealand Institute for Crop & Food Research Limited, Private Bag 11-600, Palmerston North, New Zealand;(2) Centre for Novel Agricultural Products, Biology Department (Area 8), University of York, YO10 4AD York, UK |
| |
Abstract: | Summary The regal pelargonium, ev. Dubonnet, was transformed using the disarmed Agrobacterium tumefaciens strains LBA4404 or EHA105 containing the binary vector pLN70. This plasmid carries on its T-DNA the rolC gene from Agrobacterium rhizogenes under control of the CaMV 35S promoter and the npt II selectable marker gene under a NOS promoter. Six independent transformants were produced and grouped according to their
phenotypic characteristics. Two transformants showed the same phenotype as the untransformed control plants. Three transformants
exhibited a dwarf phenotype and one displayed a super-dwarf phenotype. Southern hybridization analyses of the T-DNA left border
region using a npt II probe showed that the six transformants all arose from independent transformation events. Northern hybridization analyses
showed that the rolC gene was expressed only in the four transformants that exhibited a dwarf phenotype. Our data show that the phenotypic effects
of rolC expression in regal pelargoniums include reductions in plant height, leaf area, petal area, and corolla length. Earlier
flowering of the rolC transgenics by up to 22d was also observed. |
| |
Keywords: | Agrobacterium tumefaciens genetic transformation kanamycin resistance |
本文献已被 SpringerLink 等数据库收录! |
|