| 新疆伊犁地区马脑组织西尼罗病毒感染的调查 |
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| 引用本文： | 胡子成,冯裕星,何丰,徐红波,孙后超,展群岭,徐鸣明,胡永波,陈晓,谢鹏. 新疆伊犁地区马脑组织西尼罗病毒感染的调查[J]. 中国微生态学杂志, 2010, 22(2): 126-129 |
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| 作者姓名： | 胡子成 冯裕星 何丰 徐红波 孙后超 展群岭 徐鸣明 胡永波 陈晓 谢鹏 |
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| 作者单位： | 1. 重庆医科大学附属第一医院,神经内科,重庆,400016
2. 重庆医科大学附属第一医院,神经内科,重庆,400016;重庆医科大学,神经科学研究中心,重庆400016 |
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| 基金项目： | 国家重大科学研究计划(973)项目，国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的了解新疆伊犁地区草原放养马群中西尼罗病毒(W estN ile virus,WNV)中枢感染的流行状况。方法采用一步法实时荧光定量逆转录聚合酶链反应(Real Tim e RT-PCR)对采自新疆伊犁地区草原放养、未接种WNV疫苗的189例马脑组织进行WNV包膜蛋白(E)基因片段检测。结果被检马脑组织标本中未发现WNV E基因片段。结论目前尚没有证据表明我国新疆伊犁地区的放养马中存在WNV脑炎的感染,提示该地区出现WNV脑炎流行的可能性小。
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| 关 键 词： | 西尼罗病毒 新疆 实时RT-PCR E基因 马 病毒性脑炎 |
Detection of West Nile virus infection in brain tissue of horses in Ili,Xinjiang |
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| HU Zi-cheng,FENG Yu-xing,HE Feng,XU Hong-bo,SUN Hou-chao,ZHAN Qun-ling,XU Ming-ming,HU Yong-bo,CHEN Xiao,XIE Peng. Detection of West Nile virus infection in brain tissue of horses in Ili,Xinjiang[J]. Chinese Journal of Microecology, 2010, 22(2): 126-129 |
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| Authors: | HU Zi-cheng FENG Yu-xing HE Feng XU Hong-bo SUN Hou-chao ZHAN Qun-ling XU Ming-ming HU Yong-bo CHEN Xiao XIE Peng |
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| Affiliation: | HU Zi-cheng1,FENG Yu-xing1,HE Feng1,XU Hong-bo1,SUN Hou-chao1,ZHAN Qun-ling1,XU Ming-ming1,HU Yong-bo1,CHEN Xiao1,XIE Peng1,2(1.Department of Neurology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China,2.Institute of Neuroscience,Chongqing Medical University,China) |
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| Abstract: | Objective To detect the West Nile virus(WNV) infection in central nerve system of horses in Ili,Xinjiang.Method One step real-time RT-PCR was used to detect the WNV envelope gene fragment in 189 horses brain samples from Ili grassland,Xinjiang.Result No positive envelope gene fragment was detected in these samples.Conclusion There is no evidence to support WNV infection in horses raised on Ili grassland,Xinjiang.The result suggest that an outbreak of encephalomyelitis caused by West Nile virus would be impo... |
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| Keywords: | West Nile virus Xinjiang Real Time RT-PCR E Gene Horse Encephalitis |
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