Electrogenic Properties of the Na+,K+-ATPase Probed by Presteady State and Relaxation Studies |
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Authors: | Ernst Bamberg Ron J Clarke Klaus Fendler |
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Institution: | Max-Planck-Institut für Biophysik, Frankfurt am Main, Germany. ernst.bamberg@mpibp-frankfurt.mpg.de |
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Abstract: | Electrical measurements on planar lipid bilayers, patch/voltage clamp experiments, and spectroscopic investigations involving a potential sensitive dye are reviewed. These experiments were performed to analyze the kinetics of charge translocation of the Na+,K+-ATPase. High time resolution was achieved by applying caged ATP, voltage-jump, and stopped-flow techniques, respectively. Kinetic parameters and the electrogenicity of the relevant transitions in the Na+,K+-ATPase reaction cycle are discussed. |
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Keywords: | BLM patch clamp rate constant access channel sodium pump transport |
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