Reconstitution of human fetal hemoglobin from isolated alpha and gamma chains |
| |
Authors: | Y Kawamura-Konishi H Suzuki |
| |
Institution: | Department of Biophysical Chemistry, Kitasato University School of Medicine Kanagawa, Japan. |
| |
Abstract: | The reconstitution of hemoglobin F from isolated alpha and gamma chains was studied. An equimolar amounts of the alpha and gamma chains were mixed and incubated in 10 mM potassium phosphate buffer, pH 7.0, at 25 degrees C. Formation of hemoglobin F in the mixture was measured by separating hemoglobins on a cation exchange HPLC. Time courses of the formation of Hb F were independent of the protein concentration and could be analyzed on an exponential process with a first-order rate constant of (2.0 +/- 0.4) x 10(-3) h-1. Under the experimental conditions the isolated gamma chain existed as tetramer dominantly. These results suggest that the overall reaction of the reconstitution of hemoglobin F is limited by the dissociation step of the self-associated gamma chain. |
| |
Keywords: | |
|
|