Localisation of fucoxanthin chlorophyll a/c-binding polypeptides of the centric diatom Cyclotella cryptica by immuno-electron microscopy |
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Authors: | M Westermann E Rhiel |
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Institution: | Elektronenmikroskopisches Zentrum, Klinikum der Friedrich-Schiller-Universit?t Jena, Jena Arbeitsgruppe Geomikrobiologie, Institut für Chemie und Biologie des Meeres, Carl-von-Ossietzky-Universit?t Oldenburg, Oldenburg
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Abstract: | Antisera were raised against the C termini of three fucoxanthin chlorophyll a/c-binding polypeptides, Fcp2, Fcp4, and Fcp6, of the centric diatom Cyclotella cryptica. Immunogold electron microscopy of ultrathin-sectioned cells indicated that Fcp2 and Fcp4 are present in almost the same amounts, whereas approximately 8- to 10-fold less gold label was registered for Fcp6. Immunogold electron microscopy of freeze-fracture replicas of thylakoid membranes showed that the C termini of at least Fcp2 and Fcp4 were located in the thylakoid lumen, thus demonstrating a 3-dimensional structure similar to that already described for the chlorophyll a/b-binding light-harvesting polypeptides of higher plants. |
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Keywords: | : Diatom Cyclotella cryptica Immunogold labelling Electron microscopy Freeze fracture Light-harvesting complex |
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