AROS-29 is involved in adaptive response to oxidative stress |
| |
Authors: | Montesano Gesualdi Nicola Chirico Giuseppa Catanese Maria Teresa Pirozzi Giuseppe Esposito Franca |
| |
Affiliation: | Università di Napoli Federico II, Dipartimento di Biochimica e Biotecnologie Mediche, Naples, Italy. |
| |
Abstract: | Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress. |
| |
Keywords: | Oxidative stress gene expression diethylmaleate adaptive responses reactive oxygen species |
本文献已被 InformaWorld PubMed 等数据库收录! |
|