Syrian hamster lymphocyte cytogenetic technique and effects of blood storage on mitotic index |
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Authors: | Dorothy A. Cady John B. Mailhes |
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Affiliation: | (1) Department of Biology, Louisiana State University-Shreverport, 71115 Shreveport, Louisiana;(2) Department of Obstretics and Gynecology, Louisiana State University Medical Center, P. O. Box 33932, 71130 Shreveport, Louisiana |
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Abstract: | Summary The Syrian hamster has a diploid chromosome complement similar to humans in both number (2N=44) and morphology. For comparative mutagenic studies with humans, a repeatable lymphocyte chromosome technique involving laboratory mammals is desirable. The reported hamster lymphocyte cytogenetic technique appears technically uncomplicated and repeatable while providing a sufficient number of metaphase cells for quantiative analysis. In order to determine the effects of storing whole-blood (in culture media) for varying time periods at 8°C prior to adding a mitogen, the mitotic index was calculated following a 48-h culture period. Results indicated the storage up to six days can still result in a mitotic index adequate for cytogenetic analyses. |
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Keywords: | Syrian hamster lymphocyte cytogenetics mitotic index |
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