Metachromatic assay for the quantitative determination of bacterial endotoxins |
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| Authors: | T Keler A Nowotny |
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| Affiliation: | 1. Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India;2. Translational Research Platform for Veterinary Biologicals (TRPVB), TANUVAS, II Floor, Central University Laboratory Building, Madhavaram Milk Colony, Chennai 600051, India |
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| Abstract: | A metachromatic dye, 1,9-dimethylmethylene blue (DMB) reacts with bacterial endotoxins. This interaction results in a shift of the absorption maximum of DMB to shorter wavelengths. The findings indicate that the negatively charged lipid moiety of the endotoxic lipopolysaccharide reacts with DMB. The lowest amount of endotoxin detectable by the procedure described here is approximately one microgram. The dye could not be used in the presence of serum components. DMB mixed to column chromatographic effluent showed good resolution in continuous monitoring of endotoxin components leaving the column. |
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