Increased stable inheritance of herbicide resistance in transgenic lettuce carrying a petE promoter-bar gene compared with a CaMV 35S-bar gene |
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Authors: | M S McCabe F Schepers A van der Arend U Mohapatra A M M de Laat J B Power M R Davey |
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Institution: | (1) Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK Fax: +44(0)1159513298 e-mail: mike.davey@nottingham.ac.uk, GB;(2) Van der Have Research, P.O. Box 1, 4410 AA Rilland, The Netherlands, NL;(3) Leen de Mos Seeds, ’s-Gravenzande, P.O. Box 54-2690 AB, The Netherlands, NL |
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Abstract: | Inheritance of resistance to herbicide (300 mg/l glufosinate ammonium) up to the third (T3) seed generation was compared
in two populations of transgenic lettuce (Lactuca sativa L. cv ’Evola’) harbouring a T-DNA containing the bar gene, linked to either the Cauliflower Mosaic Virus (CaMV) 35S promoter, or a –784-bp plastocyanin promoter from pea (petE). Only 2.5% (4/163) of CaMV 35S-bar plants, selected by their kanamycin resistance(T0 generation), transmitted herbicide resistance at high frequency to their
T3 seed generation compared with 97% (29/30) for kanamycin resistant petE-bar plants. In the case of 35S-bar transformants, only 16% (341/2,150) of the first seed generation (T1) plants, 22% (426/1,935) T2 plants and 11% (1,235/10,949)
T3 plants were herbicide-resistant. In contrast, 63% (190/300) T1 plants, 83% (2,370/2,845) T2 plants and 99% (122/123) T3
petE-bar transformed plants were resistant to glufosinate ammonium. The T-DNAs carrying the petE-bar and CaMV 35S-bar genes also contained a CaMV 35S-neomycin phosphotransferase (nptII) gene. ELISA showed that NPTII protein was absent in 29% (45/156) of the herbicide-resistant T2 plants from 8/19 herbicide-resistant
petE-bar lines. This indicated specific inactivation of the CaMV 35S promoter on the same T-DNA locus as an active petE promoter. The choice of promoter and T-DNA construct are crucial for long-term expression of transgenes in lettuce.
Received: 13 November 1998 / Accepted: 20 February 1999 |
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Keywords: | bar gene CaMV 35S promoter Herbicide resistance Lettuce Pea plastocyanin promoter Transgene silencing |
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