Cell membrane fluidity related to electroporation and resealing |
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Authors: | Ma?a Kandu?er Marjeta ?entjurc Damijan Miklav?i? |
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Institution: | (1) Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, Ljubljana, Slovenia;(2) Jozef Stefan Institute, Jamova 39, Ljubljana, Slovenia |
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Abstract: | In this paper, we report the results of a systematic attempt to relate the intrinsic plasma membrane fluidity of three different
cell lines to their electroporation behaviour, which consists of reversible and irreversible electroporation. Apart from electroporation
behaviour of given cell lines the time course required for membrane resealing was determined in order to distinguish the effect
of resealing time from the cell’s ability to survive given electric pulse parameters. Reversible, irreversible electroporation
and membrane resealing were then related to cell membrane fluidity as determined by electron paramagnetic resonance spectroscopy
and computer characterization of membrane domains. We found that cell membrane fluidity does not have significant effect on
reversible electroporation although there is a tendency for the voltage required for reversible electroporation to increase
with increased membrane fluidity. Cell membrane fluidity, however, may affect irreversible electroporation. Nevertheless,
this effect, if present, is masked with different time courses of membrane resealing found for the different cell lines studied.
The time course of cell membrane resealing itself could be related to the cell’s ability to survive. |
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Keywords: | Electroporation Fluidity Order parameter Membrane domain In vitro V-79 DC-3F B16-F1 |
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