Separate DNA sequences are required for normal female and ecdysone-induced male expression of Drosophila melanogaster yolk protein 1 |
| |
Authors: | Alan D Shirras and Mary Bownes |
| |
Institution: | (1) Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JR Edinburgh, UK |
| |
Abstract: | Summary
Drosophila melanogaster flies were transformed with a yp1-Adh fusion gene with 890 bp of yp1 5 flanking sequence. In an Adh
- background these flies show a stage, tissue and sex-specific pattern of alcohol dehydrogenase (ADH) activity characteristic of yolk protein genes. ADH activity is not present in dsx
D/dsx pseudomales indicating that this fragment contains sites where the dsx gene product exerts its effect. Transformed male flies do not exhibit ADH activity when injected with 20-hydroxyecdysone while synthesis of native yolk proteins is induced. Thus the hormone inducibility and sex regulation have been separated in this construct. |
| |
Keywords: | Drosophila melanogaster Yolk proteins Gene fusion 20-Hydroxyecdysone Sex determination |
本文献已被 SpringerLink 等数据库收录! |