The effect of substituted amides of pyrazine-2-carboxylic acids on flavonolignan production in <Emphasis Type="Italic">Silybum marianum</Emphasis> culture <Emphasis Type="Italic">in vitro</Emphasis> |
| |
Authors: | Lenka Tumova Katerina Gallova Jana Rimakova Martin Dolezal Jiri Tuma |
| |
Institution: | (1) Department of Pharmacognosy, School of Pharmacy in Hradec Kralove, Charles University in Prague, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic;(2) Department of Pharmaceutical Chemistry and Drug Control, School of Pharmacy in Hradec Kralove, Charles University in Prague, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic;(3) Department of Biology, Faculty of Education, University of Hradec Kralove, Vita Nejedleho 573, 500 03 Hradec Kralove, Czech Republic |
| |
Abstract: | In vitro plant tissue and cell cultures were used to study herbicide effects on growth, selected metabolic activities and other phenomena.
The effect of abiotic elicitors, two newly synthesized substituted amides of pyrazine-2-carboxylic acids (synthesized at the
Department of Pharmaceutical Chemistry and Drug Control, School of Pharmacy in Hradec Kralove), on the flavonolignan accumulation
in callus and suspension culture of Silybum marianum (L.) Gaertn. was investigated. The compounds markedly influenced production of flavonolignans in an in vitro culture. Particularly after the elicitation with a solution of compound 3-methylamide 5-tert-butylpyrazine-2-carboxylic acid at a concentration of 3.71×10−7 mol 1−1 and within 72 h of elicitation, an increase in flavonolignan production by 893 % in suspension culture versus control took place. The flavonolignan accumulation in callus culture after the elicitation with a solution of 5-brom-2-hydroxyphenylamide
of 5-tert-butyl-6-chloropyrazine-2-carboxylic acid was also increased by about 1039% (24 hour elicitation and concentration of 2.59×10−4 mol 1−1). |
| |
Keywords: | abiotic elicitors amides of carboxylic acids culture in vitro flavonolignan milk thistle |
本文献已被 SpringerLink 等数据库收录! |
|