NMR spectroscopic study of single-stranded DNA fragments of d(CGGCGAAAGCCG) and d(CGGCAAAAGCCG). |
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| Authors: | J Tanikawa Y Nishimura I Hirao K Miura |
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| Institution: | Graduate School of Integrated Science, Yokohama City University, Japan. |
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| Abstract: | We have investigated the structures of two kinds of single-stranded DNA fragments, d(CGGCGAAAGCCG) and d(CGGCAAAAGCCG), by use of NMR spectroscopy. It was found that the former takes a hair-pin like structure stabilized by hydrogen bonding of G/C base pairs in the stem region, while the latter takes a rather extended helical structure. The stable conformation of the former DNA is considered to originate from the stability of the sequence-specific conformation of the loop region rather than the stem region. |
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