| 鹿源BVDV基因E_0蛋白特性分析 |
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| 引用本文： | 郜玉钢,李璠瑛,杨鹤,杜锐,臧埔. 鹿源BVDV基因E_0蛋白特性分析[J]. 生物学杂志, 2010, 27(5): 1-3. DOI: 10.3969/j.issn.1008-9632.2010.05.001 |
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| 作者姓名： | 郜玉钢 李璠瑛 杨鹤 杜锐 臧埔 |
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| 作者单位： | 吉林农业大学中药材学院,长春,130118 |
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| 基金项目： | 国家自然科学基金资助项目，博士后基金，博士点基金 |
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| 摘 要： | 以DNAStar5.01软件,对鹿源BVDV基因E0蛋白特性进行了分析。结果表明E0蛋白由227个残基构成,等电点为7.61。E0蛋白中有与地衣类植物核苷酸酶的保守区域相似序列,维持催化活性所必需的氨基酸残基区域在28-40和71-89处,催化活性的氨基酸为His30、His79,且该序列高度保守。E0蛋白氨基酸亲水性与抗原性指数成平行相关,BVDV各毒株间非常相似,亲水性与抗原性指数都较高的区域有8-16,23-29,59-67,70-81,96-109,114-122,127-134,137-143,165-172,186-198和213-221,适合研制亚单位基因工程疫苗。
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| 关 键 词： | 梅花鹿 牛病毒性腹泻病毒 E0基因 |
Analysis on BVDV gene of Eo protein's character in Chinese sika deer |
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| GAO Yu-gang,LI Fan-ying,YANG He,DU Rui,ZANG Pu. Analysis on BVDV gene of Eo protein's character in Chinese sika deer[J]. Journal of Biology, 2010, 27(5): 1-3. DOI: 10.3969/j.issn.1008-9632.2010.05.001 |
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| Authors: | GAO Yu-gang LI Fan-ying YANG He DU Rui ZANG Pu |
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| Affiliation: | (College of Chinese Medicine Material,Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | The BVDV gene of E0 protein′s character in sika deer was analyzed by using DNAStar 5.01software.The results showed that E0 protein was constructed with 227 residues and was 7.61 its isoionic point.E0 protein had similar sequences compared with lichens species plants′ nucleophosphatase conservative area.Its essential amino acid residue area with catalytic activity was in 28-40 and 71-89,the amino acids with catalytic activity were His30 and His79,and the sequences were high conservation.The amino acids of E0 protein′s hydrophilicity of exponent has parallel correlation compared with antigenicity,each of BVDV strain was extremely similar,and the higher numerus areas were 8-16,23-29,59-67,70-81,96-109,114-122,127-134,137-143,165-172,186-198,213-221,which were suited to development subunit genetically engineering vaccine. |
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| Keywords: | Chinese sika deer bovine viral diarrhea virus E0 gene |
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