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Nicotiana chloroplast genome
Authors:C. M. Lin  S. D. Kung
Affiliation:(1) Department of Biological Sciences, University of Maryland Baltimore County, 21228 Catonsville, MD, USA
Abstract:Summary Using the existing restriction map and probes from wheat and pea ct-DNA, seven protein genes have been localized in the chloroplast genome of N. tabacum. On the clock-like map, the location of each gene is indicated by its time zone: the 15.2 kD polypeptide of the cytochrome b/f complex at 3ratio15, cytochrome f at 4ratio30, LS of RuBPCase at 4ratio50, both beta and epsiv subunits of ATP synthase at or near 5ratio00, proton-translocating subunit of ATP synthase at 8ratio20, agr subunit of ATP synthase at 8ratio40 and the 32 kD protein at 9ratio30. The genome organization of Nicotiana chloroplast DNA is similar to spinach.
Keywords:Southern hybridization  Restriction map  Chloroplast relatedness
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