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Enhancement of neutral endopeptidase activity in SK-N-SH cells by green tea extract
Authors:MF Melzig  M Janka
Institution:1. Institut für Pharmazie, Freie Universität Berlin, Germany;2. Institut für Biologie, Humboldt-Universität zu Berlin, Germany;1. Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 103, 41125 Modena, Italy;2. Department of Chemical and Pharmaceutical Sciences, University of Ferrara Via Fossato di Mortara 17-19, Ferrara, 44100, Italy;3. Department of Pharmacy, Section of Medicinal Chemistry, School of Medical and Pharmaceutical Sciences, University of Genova, Viale Benedetto XV, 3, 16132 Genova, Italy;4. Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, P.le A. Moro 5, 00185 Roma, Italy;1. Department of Clinical Pharmacology and Therapeutics, Kyoto University Hospital, Sakyo-ku, Kyoto, Japan;2. Department of Hospital Pharmacy and Pharmacology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan;3. Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan;1. Department of Neonatology, The Second Affiliated Hospital and Yuying Children''s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China;2. Molecular Pharmacology Research Center, School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China;1. Department of Pharmacy, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, PR China;2. Chinese Herb Medicine Division, Zhejiang Agriculture and Forestry University, 88 North Circle Road, Lin’an 311300, PR China;3. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, 88 North Circle Road, Lin’an 311300, PR China;4. Safety Pharmacology Division, Olivepharmasolutions Ltd, 333 Changhong Road, Wukang 313200, PR China
Abstract:Green tea extract (EFLA85942) is able to induce specifically the neutral endopeptidase (NEP) activity and to inhibit the proliferation of SK-N-SH cells; the angiotensin-converting enzyme (ACE) activity is not influenced under the same conditions. The treatment of the cells with arabinosylcytosine and green tea extract results in a strong enhancement of cellular NEP activity whereas cellular ACE activity was not changed significantly, indicating a green tea extract-specific regulation of NEP expression. Because of its role in the degradation of amyloid beta peptides this enzyme induction of NEP by long term treatment with green tea extract may have a beneficial effect regarding the prevention of forming amyloid plaques.
Keywords:green tea  neutral endopeptidase  angiotensin-converting enzyme  enzyme induction
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