DNA interaction studies of tridentate bridged Ru(II)-Pt(II) mixed-metal supramolecules |
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| Authors: | Aaron J Prussin II Avijita Jain Karen J Brewer |
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| Institution: | a Departments of Chemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0212, USA
b Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA |
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| Abstract: | Reported herein are studies of the concentration and temperature dependent interactions with DNA of the stereochemically defined mixed-metal supramolecular complexes, (tpy)Ru(tppz)PtCl](PF6)3 and ClPt(tppz)Ru(tppz)PtCl](PF6)4 (tpy = 2,2′:6′,2′′-terpyridine and tppz = 2,3,5,6-tetrakis(2-pyridyl)pyrazine). These metal complexes couple a ruthenium based light absorber (LA) to the bioactive platinum sites (BAS) using a tridentate bridging ligand (BL). The complexes exhibit intense Ru → tppz(π∗) metal to ligand charge transfer (MLCT) transitions in the visible region and adopt a square planar geometry around the Pt(II) center. The effect of incubating these metal complexes with DNA on the subsequent migration of DNA through an agarose gel was found to be more dramatic than that observed for the well known anticancer drug, cis-Pt(NH3)2Cl2] (cisplatin). This effect was enhanced with increased incubation temperature. Unwinding of supercoiled plasmid DNA was found to be more pronounced for the trimetallic complex, ClPt(tppz)Ru(tppz)PtCl](PF6)4, than for the bimetallic complex, (tpy)Ru(tppz)PtCl](PF6)3. |
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| Keywords: | Mixed-metal Supramolecular Cisplatin DNA interaction Bioactive site Light absorber Ruthenium Platinum Tridentate Bridging ligand |
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