Towards [CpRh(bpy)(H2O)]-promoted P450 catalysis: Direct regeneration of CytC

The organometallic complex pentamethylcyclopentadienyl rhodium 2,2′-bipyridin ([Cp^∗Rh(bpy)(H₂O)]²⁺) was applied as regeneration catalyst for cytochrome C (CytC). Direct reduction of CytC-bound Fe^III was achieved in this model system pointing towards a potential usefulness of this concept to promote cell-free P450 catalysis. In addition, controlled in situ provision with hydrogen peroxide was performed using [Cp^∗Rh(bpy)(H₂O)]²⁺ resulting in improved CytC-catalyzed sulfoxidation of thioanisol This work represents the first step towards the direct-[Cp^∗Rh(bpy)(H₂O)]²⁺ catalyzed regeneration of P450 monooxygenases and peroxidases.