Development and characterization of 15 novel microsatellite markers from forest musk deer (<Emphasis Type="Italic">Moschus berezovskii</Emphasis>) |
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Authors: | Sha-Sha Zhao Xuan Chen Sheng-Guo Fang Qiu-Hong Wan |
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Institution: | (1) College of Life Sciences, Zhejiang University, Hangzhou, 310058, P.R. China;(2) State Conservation Center for Gene Resources of Endangered Wildlife and the Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Hangzhou, 310058, P.R. China |
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Abstract: | A total of 15 polymorphic microsatellite loci were isolated from forest musk deer (Moschus berezovskii). These loci showed high levels of allelic diversity, presenting 6–14 alleles per locus in a group with 31 individuals. The
mean observed and expected heterozygosities were 0.82 and 0.68, respectively. The average polymorphic information content
value, cumulative discrimination power, overall probability of exclusion with both unknown parents or with only unknown sire
for these markers were 0.78, 1.0, 0.9999 and 1.0, respectively, indicating that this suit of microsatellite loci would be
a powerful tool for the genetic determination of the forest musk deer. |
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Keywords: | Forest musk deer Microsatellite development Polymorphic information content Discrimination power Exclusion probability |
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