共聚焦荧光镜检术揭示大蒜鳞片细胞间期核中的F肌动蛋白网络

采用免疫荧光和荧光分子探针技术与共聚焦激光扫描显微镜观察相结合，对大蒜（Ａｌｌｉｕｍ ｓａｔｉｖｕｍ Ｌ．）鳞片细胞间期核中是否存在Ｆ肌动蛋白进行了研究。结果表明，以兔抗肌动蛋折抗体为一抗、ＦＴＴＧ－羊抗兔ＩｇＧ抗体为二抗进行免疫荧光标记实验，在荧光镜下观察到蒜瓣薄壁组织的细胞核及表皮细胞核均发出明亮的黄绿色荧光经共聚焦激光扫描显微镜进一步检查，整个细胞核呈黄绿色荧光，说明其中含有肌动蛋白。经ＴＲ

Revealing the F_actin Networks in Interphase Nuclei of Garlic Clove Cells by Confocal Fluorescence Microscopy

The interphase nuclei of parenchyma cells and epidermal cells of garlic ( Allium sativum L.) clove were labelled with rabbit anti_actin antibody and FITC_conjugated goat anti_rabbit IgG antibody. The authors observed results with fluorescence microscopy and confocal laser scanning microscopy. The nuclei showed prominent green_yellow fluorescence, indicating the presence of actin in the nuclei. Fluorescence examination with TRITC_phalloidin showed distinctive red fluorescence in the nuclei, indicating that F_actin is present in the nuclei. Confocal laser scanning microscopy indicated the presence of F_actin containing network structures in the nuclei, but the network structures were absent and the nuclei still showed red fluorescence when the cells were treated with cytochalasin D before fixation; the red fluorescence in the nuclei was hard to be observed when the cells were treated with unlabelled phalloidin before the cells were stained with TRITC_phalloidin. These results indicate that F_actin is in the nuclei and forms network structures in the nuclei of garlic cells.