LTW4 Protein on Mouse Chromosome 1 Is a Member of a Family of Antioxidant Proteins |
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Authors: | Olga A Iakoubova Lisa A Pacella Helen Her David R Beier |
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Institution: | Genetics Division, Brigham and Women's Hospital, Harvard Medical School, 75 Frances Street, Boston, Massachusetts, 02115 |
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Abstract: | Based on its map position, polymorphism pattern, and expression in the kidney, the gene encoding liver 20,000–30,000 MW protein 4 (LTW4) can be considered a potential candidate for theJckm2modifying locus, which mediates the severity of polycystic kidney disease in thejuvenile cystic kidneymouse. Using two-dimensional gel electrophoresis, we identified variants of a 26-kDa polypeptide that differed in their isoelectric points between the C57BL/6J and the DBA/2J inbred strains in a pattern similar to that originally described for LTW4 protein. N-terminal amino acid sequence was obtained by microsequencing analysis, and full-length clones were obtained by RT-PCR amplification and characterized. The map position of the cloned gene was determined and corresponded to that previously described forLtw4.The gene has homology to a class of proteins characterized as thiol-specific antioxidants that are protective against damage caused by oxidative stress. The murine MER5 gene is also a member of this gene family and has recently been renamedAntioxidant protein 1 (Aop1),based on its functional characterization. We therefore propose that the gene encoding LTW4 be calledAop2. |
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