Interleukin-10 is up-regulated by prolactin and serum-starvation in cultured mammary epithelial cells |
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Authors: | Kim Tae-Won Moon Hyung-Bae Kim Sun Jung |
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Institution: | Department of Biology, Dongguk University, Seoul 100-715, Korea. |
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Abstract: | The epithelial cells of the mammary gland go through a cycle of growth, differentiation, and involution during pregnancy. Recently, we found that interleukin-10 (IL-10) was induced at the involution stage and contributed to apoptosis in the mammary gland. To elucidate the role of the epithelial cells in involution, we examined IL-10 expression in an in vitro model of HC11 cells, in various culture conditions. IL-10 was weakly expressed early in growth but when the cells were induced to differentiate by insulin and dexamethasone expression increased slightly. Prolactin in combination with insulin and dexamethasone caused a further increase. To mimic apoptosis the culture was deprived of serum as well as hormones, and this resulted in a gradual increase in IL-10. In contrast with its ligand, the IL-10 receptor itself was not expressed in any conditions. We speculate that release of IL-10 from the epithelial cells recruits lymphocytes, which have IL-10 receptors on their cell membranes, and they in turn secrete death factors inducing apoptosis of the epithelial cells. |
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