Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco |
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Authors: | Guek-Eng Sim Chiang-Shiong Loh Chong-Jin Goh |
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Affiliation: | (1) Tissue Culture Laboratory, Botany Department, National University of Singapore, Lower Kent Ridge Road, 0511 Singapore, Republic of Singapore |
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Abstract: | Protoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2×106/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1 mgl–1 GA3, they developed into plant-lets. Rooted plantlets were obtained in 3 months after protoplast isolation.Abbreviations BAP Benzylaminopurine - GA3 Gibberellic acid - MT Murashige and Tucker medium (1969) - FDA Fluorescein diacetate |
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