Effect of Ni2+ and Cd2+ ions on thermally induced conformational transitions in poly(dA)-poly(dT) system |
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| Authors: | Victor N Zozulya Olga A Ryazanova Nataliya N Zhigalova and Yurii P Blagoi |
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| Institution: | (1) Department of Molecular Biophysics, B.Verkin Institute for Low Temperature Physics & Engineering of NAS of Ukraine, 47 Lenin ave, 61103 Kharkov, Ukraine;(2) Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody sq, 61077 Kharkov, Ukraine |
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| Abstract: | Effects of Ni2+ and Cd2+ ions on thermally induced conformational transitions in the poly(dA)·poly(dT) polynucleotide duplex and poly(dA)·2poly(dT)
triplex under near physiological ionic conditions were studied by measurement of UV absorption melting curves and static light
scattering intensity. The diagrams of conformational transitions in poly(dA)-poly(dT)-Me2+ systems were plotted. An aggregation in these polynucleotide systems arises at certain values of the metal ions concentration
and the temperature after the polymer dissociation into single strands. The phenomenon is conditioned by the aggregation of
poly(dA) via the interstrand cross-linking by the dication bridges. Unlike Ni2+, Cd2+ induces formation of very stable aggregates which did not disintegrate even upon cooling up to room temperature. |
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