Unequal crossing-over and gene conversion at the amplified CUP1 locus of yeast |
| |
Authors: | Juliet W. Welch Daniel H. Maloney Seymour Fogel |
| |
Affiliation: | (1) Department of Plant Biology, University of California, 94720 Berkeley, CA, USA |
| |
Abstract: | Summary Meiotic recombination was analyzed between two twelve-copy arrays of a gene amplification at theCUP1 locus ofSaccharomyces cerevisiae. Utilizing Southern analysis to identify spores with non-parental repeat arrays, we find that approximately 11% of a sample with 202 unselected tetrads possess at least one nonparental spore array. Both reciprocal and non-reciprocal changes are observed. The data suggest a model in which frequent mispairing among identical copies of the 2.0 kb repeat unit leads to the formation of unpaired loops containing integral numbers of repeat units. In this model, conversions involving the loops lead to non-reciprocal changes in arrays: about half are associated with reciprocal exchange, and net increases in repeat unit numbers occur about as frequently as net decreases. Thus, the known properties of gene conversion can account for all the segregations we observe. |
| |
Keywords: | Unequal crossing-over Gene conversion Repeated sequence |
本文献已被 SpringerLink 等数据库收录! |
|