Visualizing the infection process of Xanthomonas campestris in cabbage using green fluorescent protein |
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| Authors: | So Jae-Seong Lim Hyoung Taek Oh Eun-Taex Heo Tae-Ryeon Koh Sung-Cheol Leung Kam Tin Lee Hung Trevors Jack T |
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| Institution: | (1) Department of Biological Engineering and the Center for Advanced Bioseparation Technology, Inha University, Inchon, 402-751, Korea;(2) Division of Civil and Environmental Engineering, Korea Maritime University Pusan, 606-791, Korea;(3) Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1;(4) Present address: Department of Biology, Lakehead University, Thunder Bay, Ontario, Canada, P7B5E1 |
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| Abstract: | The plant pathogen, Xanthomonas campestris NRRL B-1459 was chromosomally tagged with gfp, and the transformant, which was subjected to Southern hybridization showed the presence of gfp in the chromosome. The virulence-related gene of the transformant was not affected by the insertion of gfp. After inoculation into cabbage plants, the infection process was visually studied in planta. Using a fluorescence microscope, the migration and distribution of gfp-labelled bacteria was visualized in real time. As the gfp-labelled cells were easily visualized from the beginning of infection, we observed a time delay of 2 days between distribution of the Xanthomonas cells in cabbage plant and the appearance of visible necrosis. |
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| Keywords: | Fluorescent microscopy green fluorescent protein (GFP) infection process Xanthomonas campestris |
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