Lectin binding profiles of SSEA-4 enriched,pluripotent human embryonic stem cell surfaces |
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Authors: | Alison?Venable Maisam?Mitalipova Ian?Lyons Karen?Jones Soojung?Shin Michael?Pierce Email author" target="_blank">Steven?SticeEmail author |
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Institution: | (1) Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA;(2) Department of Animal and Dairy Sciences, University of Georgia, Athens, Georgia, USA;(3) BresaGen Inc, Athens, Georgia, USA |
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Abstract: | Background Pluripotent human embryonic stem cells (hESCs) have the potential to form every cell type in the body. These cells must be
appropriately characterized prior to differentiation studies or when defining characteristics of the pluripotent state. Some
developmentally regulated cell surface antigens identified by monoclonal antibodies in a variety of species and stem cell
types have proven to be side chains of membrane glycolipids and glycoproteins. Therefore, to examine hESC surfaces for other
potential pluripotent markers, we used a panel of 14 lectins, which were chosen based on their specificity for a variety of
carbohydrates and carbohydrate linkages, along with stage specific embryonic antigen-4 (SSEA-4), to determine binding quantitation
by flow cytometry and binding localization in adherent colonies by immunocytochemistry. |
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