Somatic embryogenesis from chickpea (Cicer arietinum L.) inmature cotyledons: The effect of zeatin, gibberellic acid and indole-3-butyric acid |
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Authors: | Oscar Hita Carmen Lafarga Hilario Guerra |
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Institution: | (1) Departamento de Biologia Vegetal (Fisiología Vegetal), Facultad de Farmacia, Universidad de Salamanca, Avda del Campo Charro s/n, E-37007 Salamanca, Spain |
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Abstract: | Studies were conduced to test the effects of various cytokinins on somatic embryogenesis from chickpea (Cicer arietinum L.) immature cotyledons. Zeatin (13.7 μmol) added, to B5 basal medium, supplemented with 1.5 % sucrose and 0.2 μmol indole-3-acetic
acid, was the most effective cytokinin. Lobular structures obtained from cotyledons cultures were transferred to B5 basal
medium supplemented with gibberellic acid and indole-3-butyric acid at different concentrations. The most effective treatment
was B5 medium containing 14.4 μmol gibberellic acid plus 1.0 μmol indole-3-butyric acid in which 42.8 % of lobular structures
cultured formed normal somatic embryos. High conversion of embryos into plantlets (61.0–65.2 % embryos regenerated plants)
was observed when germinated embryos were placed on plant development medium. |
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Keywords: | Chickpea Cicer arietinum L Somatic embryogenesis Zeatin Gibberellic acid Indole-3-butyric acid |
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