Fluorescence microscopy of the endomembrane system of living plant cells |
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Authors: | N. HARRIS P. GATES |
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Affiliation: | University of Durham, Department of Botany, Durham DH1 3LE, U.K. |
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Abstract: | Abstract The fluorochrome Auramine O has been evaluated as a fluorescent probe for components of the endomembrane system of living plant cells. At 0.001% w/v the compound did not inhibit seedling growth or cytoplasmic streaming but stained the nuclear envelope, endoplasmic reticulum and Golgi apparatus. The three-dimensional, structural interrelationships of these organelles in living tissues could be resolved after minimal tissue preparation. The method is also a valuable control treatment for use in conjunction with electron microscope fixation procedures. It provides a rapid means of examining dynamic changes in the endomembrane system associated with cell development and differentiation and could have application in monitoring the effects of applied physiological or chemical stress. |
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Keywords: | Auramine O endomembranes fluorescence microscopy thick section electron microscopy tissue differentiation |
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