The low-molecular-weight glutenin subunit proteins of primitive wheats. IV. Functional properties of products from individual genes |
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Authors: | Y.-K. Lee F. Bekes P. Gras M. Ciaffi M. K. Morell R. Appels |
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Affiliation: | (1) CSIRO-Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia, AU |
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Abstract: | Three genes encoding the low-molecular-weight glutenin subunits (LMW-GSs), LMWG-E2 and LMWG-E4, from A-genome diploid wheat species, and LMW-16/10 from a D-genome diploid wheat, were expressed in bacteria. The respective proteins were produced on a relatively large scale and compared with respect to their effects on flour-processing properties such as dough mixing, extensibility and maximum resistance; these are important features in the end-use of wheat for producing food products. The LMWG-E2 and LMWG-E4 proteins caused significant increases in peak resistance and mixing time, compared to the control, when incorporated into dough preparations. The LMWG-16/10 protein was qualitatively less effective in producing these changes. All three proteins also conferred varying degrees of decrease in dough breakdown. LMWG-E2 and LMWG-E4 caused significant increases in dough extensibility, and decreases in maximum resistance, relative to the control. LMW-16/10 did not show a significant effect on extensibility but showed a significant decrease in maximum resistance. The refinement of relating specific features of the structure of the LMW-GS genes to the functional properties of their respective proteins is discussed. Received: 24 November 1997 / Accepted: 18 August 1998 |
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Keywords: | Low-weight glutenin subunits Single proteins extensibility Dough properties |
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