The low-molecular-weight glutenin subunit proteins of primitive wheats. IV. Functional properties of products from individual genes |
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Authors: | Y-K Lee F Bekes P Gras M Ciaffi M K Morell R Appels |
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Institution: | (1) CSIRO-Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia, AU |
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Abstract: | Three genes encoding the low-molecular-weight glutenin subunits (LMW-GSs), LMWG-E2 and LMWG-E4, from A-genome diploid wheat
species, and LMW-16/10 from a D-genome diploid wheat, were expressed in bacteria. The respective proteins were produced on
a relatively large scale and compared with respect to their effects on flour-processing properties such as dough mixing, extensibility
and maximum resistance; these are important features in the end-use of wheat for producing food products. The LMWG-E2 and
LMWG-E4 proteins caused significant increases in peak resistance and mixing time, compared to the control, when incorporated
into dough preparations. The LMWG-16/10 protein was qualitatively less effective in producing these changes. All three proteins
also conferred varying degrees of decrease in dough breakdown. LMWG-E2 and LMWG-E4 caused significant increases in dough extensibility,
and decreases in maximum resistance, relative to the control. LMW-16/10 did not show a significant effect on extensibility
but showed a significant decrease in maximum resistance. The refinement of relating specific features of the structure of
the LMW-GS genes to the functional properties of their respective proteins is discussed.
Received: 24 November 1997 / Accepted: 18 August 1998 |
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Keywords: | Low-weight glutenin subunits Single proteins extensibility Dough properties |
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