Field planting of alfalfa artificial seeds |
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Authors: | Jo Ann A Fujii David Slade Jaime Aguirre-Rascon Keith Redenbaugh |
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Institution: | (1) Calgene, Inc., 1920 Fifth Street, 95616 Davis, California;(2) Present address: Graduate School of Business, The University of Chicago, 60637 Chicago, IL;(3) Present address: University of Washington, 98133 Seattle, WA;(4) Present address: 1605 Elm Street, 79930 El Paso, TX |
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Abstract: | Summary Encapsulated somatic embryos (artificial seeds) and naked (uncoated) somatic embryos of alfalfa (Medicago sativa L.) were planted directly into the field to demonstrate the feasibility of using artificial seeds for direct sowing. Various
row coverings that provided protection for the somatic embryos during conversion (plant formation) in the field and encapsulation
methods were investigated. The highest conversion obtained in the field was 25% when naked somatic embryos were planted under
the protective covering of inverted styrofoam cups. In comparison, 60% conversion was obtained when embryos were planted in
potting mix in a growth chamber. Somatic embryos encapsulated by the thin-coat method converted at 23% under cups in the field
and 40% in potting mix in the growth chamber. Naked somatic embryos had an average of 13 and 9% conversion in the field under
plastic and cloth coverings, respectively, whereas encapsulated embryos converted at 5 and 14%, respectively. Direct-planted
embryos (no row covering) converted at 1% in the field. Successful conversion of coated and naked somatic embryos planted
in the field supports the concept of artificial seeds serving as a substitute for natural seeds. |
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Keywords: | Medicago conversion embryogenesis encapsulation somatic embryo |
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