|
|||||
|
|
Expression of a Modified Cry1Ie Gene in E. coli and in Transgenic Tobacco Confers Resistance to Corn Borer |
|
| Authors: | Yun-Jun LIU Fu-Ping SONG Kang-Lai HE Yuan YUAN Xiao-Xia ZHANG Peng GAO Jian-Hua WANG and Guo-Ying WANG State Key Laboratory for Agrobiotechnology National Center for Maize Improvement China Agricultural University Beijing China State Key Laboratory for Biology of Plant Diseases Insect Pests |
| Affiliation: | Yun-Jun LIU,Fu-Ping SONG1,Kang-Lai HE1,Yuan YUAN,Xiao-Xia ZHANG,Peng GAO,Jian-Hua WANG,and Guo-Ying WANG * State Key Laboratory for Agrobiotechnology,National Center for Maize Improvement,China Agricultural University,Beijing 100094,China, 1State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,CAAS,Beijing 100094,China |
| Abstract: | The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer. |
| Keywords: | Bacillus thuringiensis Cry1Iem transgenic tobacco insect-resistance |
| 本文献已被 CNKI 等数据库收录！ | |