A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion ( Allium cepa L.) |
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Authors: | Engelke T Terefe D Tatlioglu T |
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Affiliation: | Abteilung Angewandte Genetik, Universit?t Hannover, Herrenh?user Strasse 2, D-30419 Hannover, Germany. thomas.engelke@genetik.uni-hannover.de |
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Abstract: | The chimerical mitochondrial CMS(1)-specific sequence in chives ( Allium schoenoprasum) was used to develop a PCR-marker that distinguishes both male-sterility inducing cytoplasms, CMS-(S) and CMS-(T), from the normal cytoplasm in onion ( Allium cepa). In combination with a previously described marker for CMS-(S), which anchors in the upstream region of the mitochondrial gene cob, all of the three known cytoplasms in the onion are distinguishable. The PCR-marker system was tested in 361 onion plants, which were selected from F(1)-hybrids and different open-pollinated varieties. The latter are mainly landraces from Turkey, in which all three cytoplasm types were detected. |
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