Nuclear and mitochondrial DNA have sequence homology with a chloroplast gene |
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Authors: | Dara L Whisson N Steele Scott |
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Institution: | (1) CSIRO Division of Horticultural Research, Box 350 GPO, 5001 Adelaide, South Australia |
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Abstract: | Summary A PstI 7.7 kbp fragment from chloroplast (ct) DNA of spinach shows homology to an EcoRI 8.3 kbp fragment of mitochondrial
(mt) DNA and in turn, both are homologous to a number of common regions of nuclear (n) DNA. The common area of homology between
the chloroplast and mitochondrial fragments is between a KpnI 1.8 segment internal to the PstI sites in the ctDNA and an EcoRI/BamHI
2.9 kbp fragment at one end of the mitochondrial 8.3 kbp fragment. The KpnI 1.8 kbp ctDNA fragment is within a structural
gene for the P700 chlorophyll a apoprotein. Further analysis of this KpnI 1.8 kbp fragment confined the homologous region in mtDNA to a ct
0.8 kbp HpaII fragment. These smaller pieces of the organellar genomes share homologies with nuclear DNA as well as displaying
unique hybridization sites. The observations reported here demonstrate that there is a common or closely related sequence
in all three genetic compartments of the cell. |
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Keywords: | chloroplast DNA DNA homologies mitochondrial DNA nuclear DNA photosystem I spinach |
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