红豆杉细胞培养产物提取策略及其对紫杉醇的影响

研究了硫酸铈铵及原位提取对红豆杉细胞悬浮培养过程中细胞生长、紫杉醇合成及释放的影响。红豆杉细胞悬浮培养过程中培养第12d添加2mg/L硫酸铈铵能获得最大紫杉醇产量8．3mg/L，其中2．4mg/L释放到细胞外，分别为对照组的4倍及12倍。同时添加2mg/L硫酸铈铵、5％油酸(v/v)时胞外紫杉醇产量达到9mg/L，为对照组的45倍。将硫酸铈铵及原位提取与补料培养相结合，最高紫杉醇产量可达24．5mg/L，其中60％释放到胞外。

Influence of Taxol Production by Product Extraction Strategies in Cell Cultures of Taxus chinensis

The effects of product release and in situ extraction on cell growth and taxol release and accumulation in cell suspension cultures of Taxus chinensis were investigated. Addition of 2 mg/L ammonium cerous sulphate on day 12 gave the greatest taxol production at 8 3 mg/L, among which 2 4 mg/L was released out of the cell, which was 4 and 12 times that of the control culture. Extracellular taxol production reached 9 mg/L when 2 mg/L ammonium cerous sulphate and 5% oleic acid (v/v) were added together, which was 45 times that of the control culture. The highest taxol accumulation of 24.5 mg/L with the release ratio of 60% was obtained when 2 mg/L ammonium cerous sulphate, 5% oleic acid (v/v) and feeding solution with 20 g/L sucrose were added.