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| 培养法和免培养法联合检测油藏环境烃降解菌和产甲烷菌群多样性 | |
| 引用本文： | 李辉,林匡飞,牟伯中,张卫,顾继光,李洋洋.培养法和免培养法联合检测油藏环境烃降解菌和产甲烷菌群多样性[J].微生物学通报,2011,38(1):21-28. |
| 作者姓名： | 李辉 林匡飞 牟伯中 张卫 顾继光 李洋洋 |
| 作者单位： | 1. 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海,200237 2. 华东理工大学应用化学研究所,生物反应器国家重点实验室,上海,200237 3. 暨南大学生命科学技术学院,广东,广州,510632 4. 上海化工研究院,上海,200062 |
| 基金项目： | 国家自然科学基金项目(No. 50744016, 40871223, 40901148, 41003031); 中国博士后科学基金特别资助项目(No. 200902223); 水体污染控制与治理科技重大专项项目(No. 2009ZX071001-009-03); 国家高技术研究发展计划项目(No. 2007AA06Z331); 中央高校基本科研业务费专项资金资助(No. WB0911011, WB1014004) |
| 摘 要： | 烃降解菌和产甲烷菌是油藏环境微生物生态系统中重要的功能菌群, 采用DGGE和FISH方法分析了不同油藏样品中两类菌群的多样性和产甲烷活性。DGGE结果表明, 不同水样的alkB基因多样性相差较大, 而且注水井条带明显多于采油井。FISH结果表明, 油藏水样中产甲烷菌含量明显高于烃降解菌, 且两者空间分布的位置较近; 说明油藏环境中烃降解菌和产甲烷菌结成一定的相互关系。富集培养表明, 胜利油田产出液接种物培养130 d后, 石油烃降解率达到50%以上, 产甲烷的最大速率达到1.57×10?2 mmol/(L?d)。利用分子生物学方法分析油藏环境功能菌群的多样性, 可以为开展微生物采油技术的应用提供有用信息。 |
| 关 键 词： | 油藏 分子生态 微生物多样性 DGGE指纹图谱 FISH杂交 |
| 收稿时间： | 2010/6/18 0:00:00 |
| 修稿时间： | 2010/11/9 0:00:00 |
Culture-dependent and culture-independent characterization of alkane-degrading bacteria and methanogens consortium diversity associated with petroleum reservoir |
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| LI Hui,LIN Kuang-Fei,MU Bo-Zhong,ZHANG Wei,GU Ji-Guang and LI Yang-Yang.Culture-dependent and culture-independent characterization of alkane-degrading bacteria and methanogens consortium diversity associated with petroleum reservoir[J].Microbiology,2011,38(1):21-28. | |
| Authors: | LI Hui LIN Kuang-Fei MU Bo-Zhong ZHANG Wei GU Ji-Guang and LI Yang-Yang |
| Institution: | State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China;Shanghai Research Institute of Chemical Industry, Shanghai 200062, China |
| Abstract: | The alkane-degrading bacterium and methanogenic were the important functional populations in oil reservoirs and their diversities were investigated by FISH and DGGE, respectively. The DGGE profiles showed great difference between the alkB genes in samples. And the major bands detected in injection wells were obviously more than them in production wells. The FISH results indicated that the quantity of archaea was much more than bacteria in the samples and they had close spatial distribution in situ. The results indicated that alkane-degrading bacterium and methanogenic populations were diverse in oil reservoirs and formed compact aggregates in situ. The incubation of Shengli oilfield production water degraded more than 50% residual oil during 130 d culture, with the production rate of methane reaching up to 1.57×10?2 mmol/(L?d). Molecular analysis of the functional populations of microbial community in oilfield will provide the information for better application of microbial enhanced oil recovery. |
| Keywords: | Petroleum reservoir Molecular ecology Microbial diversity DGGE FISH |
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