Cutting edge: human B cell function is regulated by interaction with soluble CD14: opposite effects on IgG1 and IgE production |
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Authors: | Arias M A Rey Nores J E Vita N Stelter F Borysiewicz L K Ferrara P Labéta M O |
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Affiliation: | Department of Medicine, University of Wales, College of Medicine, Cardiff, United Kingdom. |
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Abstract: | The mechanism(s) controlling activation of naive B cells, their proliferation, Ag receptor affinity maturation, isotype switching, and their fate as memory or plasma cells is not fully elucidated. Here we show that between 24 and 60% of CD19+ cells in PBMC bind soluble CD14 (sCD14). Tonsillar B cells also bind sCD14, but preferentially the CD38-ve/low cells. Interaction of sCD14 with B cells resulted in higher levels of IgG1 and marked inhibition of IgE production by activated tonsillar B cells and Ag-stimulated PBMC. We found that sCD14 interfered with CD40 signaling in B cells, inhibited IL-6 production by activated B cells, and increased the kinetics and magnitude of CD40 ligand expression on T cells. Together with the previously reported effects on T cells, these findings define sCD14 as a novel soluble regulatory factor capable of modulating cellular and humoral immune responses by interacting directly with T and B cells. |
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