Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis |
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| Authors: | Zhao-jun Ding T. Wang Kang Chong Shunong Bai |
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| Affiliation: | (1) Research Center for Plant Molecular and Developmental Biology, Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincum, Xiangshan, Beijing 100093, China e-mail: twang@ns.ibcas.ac.cn Fax: +86-10-82594821, CN;(2) Department of Developmental Biology, Peking University, Beijing 100871, China, CN |
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| Abstract: | Yeast DMC1 is a meiosis-specific gene required for homologous chromosome pairing in meiosis. Using degenerate primers designed according to amino acid motifs conserved in yeast Dmc1 and Arabidopsis AtDmc1, we obtained full-length cDNA of a rice homologue of the DMC1 gene (OsDMC1) by RT-PCR and rapid amplification of cDNA ends (RACEs). OsDmc1 exhibited 53% amino acid sequence identity to yeast Dmc1 and 81% to AtDmc1. OsDMC1 was expressed at high-levels in reproductive organs, low-levels in roots, and undetectable levels in leaves and seedlings. Southern blot analyses revealed that OsDMC1 is one of two DMC1 homologues present in rice. Received: 18 December 2000 / Accepted: 22 December 2000 |
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| Keywords: | Meiosis Rice DMC1 |
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