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Substrate selectivity of imidazole-appended dimethyl-β-cyclodextrin |
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| Authors: | Hiroshi Ikeda Tsukasa Ikeda and Fujio Toda |
| Institution: | Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 227, Japan |
| Abstract: | The substrate selectivity of the hydrolysis reaction by imidazole-appended dimethyl-β-cyclodextrin (2) was studied using different kinds of p-nitrophenyl esters of amino acids. The tendency for the hydrolysis reaction of the amino acid ester by 2 reflected a difference in Km rather than in kcat. 2 had the ability to undergo a stereo-selective hydrolysis reaction. |
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