Sodium-dependent short-circuit current across the yolk sac membrane during embryonic development in normal and shell-less cultured chicks |
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Authors: | Makoto Takada Nancy B Clark |
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Institution: | (1) Department of Physiology, Saitama Medical School, Moroyama, Iruma-gun, 350-04 Saitama, Japan;(2) Department of Physiology and Neurobiology, University of Connecticut, U-42, 75 North Eagleville Road, 06269-3042 Storrs, CT, USA |
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Abstract: | Summary The transepithelial electrical characteristics of the isolated yolk sac membrane of normal in ovo or shell-less cultured chick embryos were investigated. In normal chicks the potential difference (blood side positive relative to yolk side) and short-circuit current of the membrane increased during development. Ouabain (10-4
M) on the blood side (basolateral side, serosal side) significantly decreased potential difference and short-circuit current but was without effect on the yolk side (brush border side, mucosal side). Substitution of choline for Na+ in the bathing solutions abolished the potential difference and the short-circuit current; when Na+ replaced choline this effect was reversed. Amiloride added to both sides of the yolk sac membrane had no effect on potential difference or short-circuit current. Injection of aldosterone (50 g) and T3 (10 M) into yolk did not induce amiloride sensitivity. The short-circuit current was not altered by addition of either glucose or alanine to the bath. The short-circuit current of the yolk sac membrane of shell-less cultured embryos was significantly lower than that of normal controls. Addition of Ca2+ to the serosal bathing medium did not reverse the foregoing condition, but decreased the short-circuit current. It is concluded that the yolk sac short-circuit current is Na+ dependent and increases with developmental age in the chick embryo.Abbreviations Hepes
N-2-hydroxyethylpiperazine-N-2-ethaneoulphonic acid
- PD
potential difference
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R
resistance
- SCC
short-circuit current
- TRIS
tris-hydroxymethyl aminomethane
- T3
3,3-5-triiodo-l-thyronine |
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Keywords: | Chick embryo Yolk sac membrane Na+-dependent SCC Amiloride insensitive SCC Shell-less culture |
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